标题: Citrobacter arsenatis sp. nov., an arsenate-reducing bacterium isolated from freshwater sediment

作者: Wang, HL (Wang, Hanlin); Hou, HB (Hou, Haobo); Huang, JX (Huang, Jiexun)

来源出版物: ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY DOI: 10.1007/s10482-021-01601-y 提前访问日期: JUN 2021

摘要: A novel arsenate-reducing bacterium, LY-1(T), was isolated from freshwater sediment in Huangshi, China. Morphological analysis indicated that the cells were shaped like rods and were gram-negative. The major fatty acids (> 10%) were C-16:0, summed feature 3 (C-16:1 omega 7c, C-16:1 omega 6c) and summed feature 8 (C-18:1 omega 7c, C-18:1 omega 6c). An assessment of the phylogeny based on 16S rRNA gene sequences indicated that the strain LY-1(T) belonged to the genus Citrobacter, while further analysis based on the recN gene indicated that LY-1(T) occupies a distinct phylogenetic niche within the Citrobacter genus. Moreover, average nucleotide identity and digital DNA-DNA hybridization between the strain LY-1(T) and the type strains of closely related species of the genus Citrobacter (C. europaeus, C. brakii, C. portucalensis, C. freundii, C. werkmanii, C. cronae, C. youngae, C. pasteurii, C. tructae, C. gillenii, and C. murliniae) were 85.8-93.8% and 31.2-56.9%, respectively. In addition, the LY-1(T) strain's capacity to metabolize various compounds and its characteristic G + C content of 51.9% were also distinct from other species of the Citrobacter genus. These discriminatory features cumulatively indicate the LY-1(T) strain as a new species within the Citrobacter genus. We propose the species name Citrobacter arsenatis for this new species, with LY-1(T) (= CCTCC AB 2019169(T) = KCTC 72440(T)) as the type strain.

入藏号: WOS:000660421100001

PubMed ID: 34117562

语言: English

文献类型: Article; Early Access

作者关键词: ANI; Arsenate; Citrobacter arsenatis; dDDH; recN

地址: [Wang, Hanlin; Hou, Haobo] Wuhan Univ, Sch Resource & Environm Sci, Wuhan, Peoples R China.

[Wang, Hanlin; Huang, Jiexun] Hubei Polytech Univ, Sch Environm Sci & Engn, Hubei Key Lab Mine Environm Pollut Control & Reme, Huangshi, Hubei, Peoples R China.

通讯作者地址: Hou, HB (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Wuhan, Peoples R China.

Huang, JX (通讯作者)，Hubei Polytech Univ, Sch Environm Sci & Engn, Hubei Key Lab Mine Environm Pollut Control & Reme, Huangshi, Hubei, Peoples R China.

电子邮件地址: hhb-bhh@163.com; huangjiexun@hbpu.edu.cn

影响因子：2.674