1.标题: Induced and natural moss soil crusts accelerate the C, N, and P cycles of Pb-Zn tailings

作者: Liao, KJ (Liao, Kejun); Tao, Y (Tao, Yue); Tu, JW (Tu, Jiawei); Zeng, YY (Zeng, Yuyang); Li, Y (Li, Yan); Wang, PP (Wang, Panpan); Li, XY (Li, Xinyue); He, F (He, Fan); Chen, LZ (Chen, Lanzhou)

来源出版物: SCIENCE OF THE TOTAL ENVIRONMENT 卷: 909 文献号: 168657 DOI: 10.1016/j.scitotenv.2023.168657 提前访问日期: NOV 2023 出版年: JAN 20 2024

摘要: Nutrient deficiency is the primary obstacle in tailing ecological restoration besides high heavy metal content. Biological soil crusts (BSCs) are known for their C and N fixation capabilities and play a crucial role in soil P cycle. BSCs are widespread in tailings and provide a potential ecological restoration approach. In 2022, we carried out an on-site restoration on a Pb-Zn tailing pond in Yunnan Province, China. BSCs were propagated by natural moss crust fragment inoculation. The induced moss crusts (IMCs) were monitored at 0, 45, 90, and 135 days and compared with natural moss crusts (NMCs). The chlorophyll-a content and abundance of biotic organisms increased over time, reaching a peak at 135 days and surpassing that of NMCs. Moss crusts increased the content of C, N, and P nutrients and enzyme activities in the 0.5 cm surface soil. They also reduced the DTPAextractable Pb content. Moss crusts significantly increased the content of fulvic/humic and protein-like/ polyphenol substances, thereby raising the humic index of soil dissolved organic matter (especially NMCs). Furthermore, moss crusts also raised the abundance of nitrification (AOB and Nsr), denitrification (narG, napA, qnorB, and nosZ), and P-cycling (gcd, appA, phoC, phoA, and phoD) genes, especially IMCs after a 135-day inoculation. NMCs exhibited higher moss abundance measured via eukaryotic photoautotrophs. Moss crusts increased photosynthetic bacteria abundance (e.g., Leptolyngbya and Nostocales) and reduced the dominance of chemoautotrophic bacteria, especially the dark sulfide oxidation bacteria (Betaproteobacteriales). This trend was more pronounced in NMCs. Overall, IMCs can recover the functions of NMCs, and in some cases (e.g., abundance and diversity of biotic community, soil nutrient and N & P cycle genes), even surpass them. Our research provides new insights into the tailing ecological restoration.

作者关键词: Biological soil crusts; Tailing ecological restoration; Dissolved organic matter; N and P cycle genes; Microbial community

2. 标题: A feasible method of induced biological soil crust propagation through the inoculation of moss and addition of soil amendments in a Pb-Zn tailing pond

作者: Liao, KJ (Liao, Kejun); Tao, Y (Tao, Yue); Zeng, YY (Zeng, Yuyang); Tu, JW (Tu, Jiawei); She, SJ (She, Sijia); Fu, YJ (Fu, Yaojia); Hou, LH (Hou, Lianghui); Chen, LZ (Chen, Lanzhou)

来源出版物: SCIENCE OF THE TOTAL ENVIRONMENT 卷: 910 文献号: 168569 DOI: 10.1016/j.scitotenv.2023.168569 提前访问日期: NOV 2023 出版年: FEB 1 2024

摘要: The stacking of tailings results in serious environmental pollution and plant growth difficulty. However, moss and microorganisms can successfully colonize in tailings to form biological soil crusts (BSCs) and provide a feasible means to ecologically restore tailing reservoirs. Nonetheless, information on this approach is scarce. In this study, a 90 day field experiment was conducted to form BSCs in a Pb-Zn tailing pond in Jianshui County, China by inoculating in-situ moss crust fragments and adding three soil amendments. Results showed that induced BSCs successfully propagated, and the biomass increased to 15.51-20.33 times the initial value. Moss inoculation considerably increased the soil moisture, water-holding capacity, and phosphatase by 9.2 %, 8.8 %, and 64.0 %, respectively, and decreased exchangeable fraction Pb by 30.7 %. The co-inoculation of moss and biochar remarkably increased soil moisture, water-holding capacity, cation exchange capacity, sucrase, urease, and phosphatase activity by 22.3 %, 23.4 %, 116 %, 80.5 %, 28.6 %, and 240 %, respectively, and decreased the bulk density by 13.3 %. The addition of red soil reduced the total contents of Pb and Zn, whereas that of the stabilizer increased the pH and decreased the bioavailability of Pb and Zn. Co-inoculation greatly increased the biotic community species richness and changed their structure and function. The dominant photosynthetic eukaryotes shifted from Synechococcales to Oscillatoriales. Bacterial nutritional types shifted from chemoautotrophy to photoautotrophy and chemoautotrophy, and fungal nutritional types changed from oligotrophy to copiotrophy. These changes drove alterations in bacterial and fungal community structures. These results indicated that the propagation of induced BSCs can rapidly improve the soil structure and nutrient cycle, restore the biotic abundance and function, and facilitate the soil formation of tailings. Thus, this method holds promise for the ecological restoration of tailings.

作者关键词: Tailing ecological restoration; Induced biological soil crusts; Soil amendments

地址: [Liao, Kejun; Tao, Yue; Tu, Jiawei; Zeng, Yuyang; Li, Yan; Wang, Panpan; Li, Xinyue; He, Fan; Chen, Lanzhou] Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

通讯作者地址: Chen, LZ (通讯作者)，Wuhan Univ, Sch Resource & Environm Sci, Wuhan 430079, Peoples R China.

电子邮件地址: chenlz@whu.edu.cn

影响因子：9.8